MPI Research has a team of scientists and highly trained technical staff available to conduct integrated research programs. In drug discovery and preclinical safety assessment, morphological changes in cells/tissues are often accompanied by alterations in DNA, RNA, or protein molecules. A further understanding of these molecular changes can be helpful in the understanding of target distribution and pharmacology and elucidation of mechanism(s) of toxicity or efficacy. Furthermore, in situ techniques, alone or in concert with molecular imaging tools, can provide more detailed information on the biodistribution of cellular therapeutic agents and biologics. MPI Research continuously expands our molecular pathology services, validates new tissue related assays, and updates equipment to anticipate and meet our Sponsors’ needs and scientific interests.
Immunohistochemistry and Immunofluorescence
Immunohistochemistry and immunofluorescence are special pathology techniques applicable for discovery and development projects. Immunohistochemistry employs antibodies to identify distinct antigens within tissues or cellular preparations. It is a highly specific tissue directed method for identifying particular cells, cell components, or molecules of interest beyond what routine histochemical staining tools. Antibodies can often target structural proteins and various soluble proteins such as enzymes, hormones, immunoglobulins, cytokines, and neurotransmitters. This sensitive and specific identification method can provide important information about test article effects and target distribution in animal models. Such information can be a useful to understand efficacy or adverse effects in animal models. Various chromogens or immunoflourescent tags can be used for antibody localization within tissues and can be tailored to client needs or technique requirements. Double staining methods and detection techniques are also possible. Our team that will work with sponsors to explore potential applications in discovery or special studies designed to help address scientific needs.
Probe Hybridization Labeling (CISH—Chromogenic In Situ Hybridization and FISH—Fluorescent In Situ Hybridization)
In situ hybridization techniques use molecular probes to detect particular specific DNA or RNA sequences. Labeled probes allow detection and localization of specific RNA and DNA sequences within cells/tissues, providing important information about test article targets, biologic effects, and potential understanding for pathogenic mechanisms for tissue changes. Our team can provide an assessment of appropriate in situ hybridization techniques and assessments to explore application to address your special needs.