Drug and Noise Induced Hearing Loss Models
Our test-retest data and interrater reliability, with expert electrophysiologists, in the field demonstrate our high level of expertise in auditory brainstem response data interpretation.
Establishing drug efficacy can only be achieved once you have a validated experimental model. MPI Research offers several systemic and intratympanic drug-induced hearing loss models using aminoglycosides, diuretics, and platinum based chemotherapeutics.
ABR threshold data in guinea pigs following intratympanic administration of gentamicin and saline.
Guinea pig ABR data presented over time following a single 122 dB exposure to 8 kHz octave band noise for 2 hours.
Noise-induced hearing loss models require specialized noise chambers, speakers, and stimulus generators. MPI Research offers a qualified method for noise induced hearing loss in both mice and guinea pigs.
Average ABR and paired cytocochleogram data from mice following a single 118 dB exposure to an 8–16 kHz bandwidth noise for two hours.
Inner Ear Immunohistochemistry (IHC)
MPI Research offers multiple IHC markers for hair cell and synapse identification. Our standard technique for inner and outer hair cell identification uses a phalloidin based marker which stains all F-actin in cells allowing for visualization of stereocilia and hair cell body both within the organ of Corti and vestibular epithelium of the utricle and saccule (available in all species). Additionally, we offer a myosin Viia antibody marker which allows for the visualization of both inner and outer hair cell bodies (available in mouse only).
Characterizing the number of neuron synaptic connections to a regenerated or traumatized hair cell can elucidate and support functional audiometry data. Using antibody markers for presynaptic and postsynaptic markers, MPI Research offers a quantitative approach to synaptic contacts on inner hair cells.