Molecular Pathology

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Molecular Pathology 2017-07-04T10:23:29+00:00
molecular pathology

MPI Research has a team of scientists and highly trained technical staff available to conduct integrated research programs. In drug discovery and preclinical safety assessment, morphological changes in cells/tissues are often accompanied by alterations in DNA, RNA, or protein molecules. A further understanding of these molecular changes can be  helpful in the understanding of target distribution and pharmacology and elucidation of mechanism(s) of toxicity or efficacy. Furthermore, in situ techniques, alone or in concert with molecular imaging tools, can provide more detailed information on the biodistribution of cellular therapeutic agents and biologics. MPI Research continuously expands our molecular pathology services, validates new tissue related assays, and updates equipment to anticipate and meet our Sponsors’ needs  and scientific interests.

Immunohistochemistry and Immunofluorescence

Immunohistochemistry and immunofluorescence are special pathology techniques  applicable for discovery and development projects. Immunohistochemistry employs antibodies to identify distinct antigens within tissues or cellular preparations.  It is a highly specific tissue directed method for identifying particular cells, cell components, or molecules of interest beyond what routine  histochemical staining tools.  Antibodies can  often target structural proteins and various soluble proteins such as enzymes, hormones, immunoglobulins, cytokines, and neurotransmitters. This sensitive and specific identification method can provide important information about test article effects and target distribution in animal models.  Such information can be a useful to understand efficacy or adverse effects in animal models.  Various chromogens or immunoflourescent tags can be used for antibody localization within tissues and can be tailored to client needs or technique requirements.  Double staining methods and detection techniques are also possible.  Our team that will work with sponsors to explore potential applications in discovery or special studies designed to help address scientific needs.

Probe Hybridization Labeling (CISH—Chromogenic In Situ Hybridization and FISH—Fluorescent In Situ Hybridization)

In situ hybridization techniques use molecular probes to detect particular specific DNA or RNA sequences. Labeled probes allow  detection and localization of specific RNA and DNA sequences within cells/tissues, providing important information about test article targets, biologic effects, and potential understanding for pathogenic mechanisms for tissue changes. Our team can provide an assessment of appropriate in situ hybridization techniques and assessments to explore application to address your special needs.

Services and Capabilities

  • Numerous validated stains across multiple research animal species and the training and expertise to develop customized IHC methods to meet unique client requirements
  • Customized method development of immunolabeling protocols for new markers
    and species
  • Demonstration of molecular targets in paraffin-embedded or frozen samples
  • Single or dual staining procedures
  • Fluorescent detection methods (with dual staining) to identify co-localizing markers
  • Immunolabeling of biopharmaceuticals, such as antibodies and therapeutic cells, and evaluation of their biodistribution
  • Quantitative and qualitative image analysis and interpretation
  • Detection of exogenous/endogenous nucleotide sequences
  • In situ demonstration of microRNA biomarkers
  • Conversion of glass slide sections into high-quality whole slide digital images
    (virtual/digital slides)
  • High resolution brightfield and fluorescent imaging

Equipment

Can perform up to 90 individualized staining protocols simultaneously, providing maximum optimization efficiency and staining throughput.
Slide scanning allows for immediate and secure slide review. Slide discussions are easily facilitated, eliminating the need for shipping glass slides. Fluorescent images are GLP-compliant, providing a permanent record.
Analysis of either brightfield or fluorescent images is a valuable tool to provide quantifiable results for a range of applications, including length and area quantification, cell counting, and microvessel analysis. Customized algorithms can be created to accommodate project specific parameters.