In Vivo Research

///In Vivo Research
In Vivo Research 2017-07-04T10:25:20+00:00

In Vivo Models

Failure is a fact of life in drug discovery. The ability to fail early, before expensive clinical trials are initiated, is an important strategic strength for any drug development company. Some research questions require the inherent complexity of a whole organism to answer, and perhaps no step before clinical trials is more important than in vivo studies. These studies help to establish the risk/benefit ratio for the potential drug, and subsequently whether it is suitable for testing in patients. Therefore, study design, execution, and interpretation are challenging and require years of experience to perform well. This complexity is only increasing, as we gain a deeper understanding of the cellular and molecular processes underlying disease, improve on existing animal models, and expand in vivo work to include multiple disciplines that were traditionally separated. MPI Research responds to the challenge with

  • Collaborative custom model development by experienced, knowledgeable scientists and Study Directors from relevant disciplines
  • An established institutional animal care and use process to facilitate and expedite approval and implementation of new or adapted models
  • A facility certified by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)

Service highlights

  • Cell- and gene-based therapeutic models (small and large animals)
  • Surgical delivery of cell-, plasmid-, and viral vector-based test articles to selected anatomic locations (small and large animals)
  • Extensive experience with immunocompromised rodents

Tumorigenicity and biodistribution of cell- and DNA-based test articles

Molecular imaging capabilities for test article assessment and biodistribution

  • Mouse peritonitis (MRSA, MSSA, Streptococcus pneumoniae, PRSP, PSSP)
  • Chinchilla acute otitis media (Haemophilus influenzae)
  • Rabbit otitis externa (Pseudomonas aeruginosae)
  • Vaccine safety studies (rodents, ferrets, canines)
  • BSL-2 laboratory and vivarium space
  • Atherosclerosis models in a variety of species (denudation and high-fat diet)
  • Surgically-induced
  • Acute myocardial infarction (ischemia/reperfusion)
  • Congestive heart failure
  • Chronic cardiac and peripheral ischemia
  • Peripheral arterial disease
  • Thrombosis models
  • Surgical models available for
    • Vascular aneurysm
    • Arteriovenous (AV) fistula
    • AV graft
    • Atrial fibrillation
    • Myocardial oxygen consumption
    • Evaluation of cardiac function – left ventricle (LV) and systemic hemodynamics, pulmonary venous (PV) loops, echocardiography
    • Off-pump cardiac surgery
  • Joint/cartilage models
  • Articular cartilage repair
  • ACL repair
  • Meniscal repair
  • Reconstruction/fixation of ligaments/tendons
  • Rotator cuff repair
  • ACL transection/joint de-stabilization model of OA
  • Hip and knee replacement
  • Calvaria critical size defect
  • Tibial/femoral/ulnar osteotomy models
  • Femoral condyle and diaphyseal defects
  • Maxillofacial/mandibular defects
  • Autograft/allograft/bone graft substitutes
  • Bones ingrowth for joint replacement
  • Non-weight bearing (axial, transcortical)
  • Bone cement, putty, etc.
  • Bacterial infection models
  • Spinal surgery/bone reconstruction
  • Posterolateral/interbody fusion
  • Intervertebral disc reconstruction
  • Discectomy/disc replacement
  • Spine intervention
  • Serial CSF collection methodology (rats)
  • Cecectomized model of diarrheal disease (PGE2, rats)
  • PLP-induced experimental autoimmune encephalomyelitis (EAE; mice)
  • Collagen-induced arthritis (rats)
  • Adjuvant-induced arthritis (monoarthritis, rats)
  • Cyclophosphamide-induced neutropenia (rodents)
  • Surgical-induced disease models
  • Growth hormone-dependent hypophysectomized model (rats)
  • Hyperparathyroidism (partial nephrectomized rats)
  • Heidelberg Spectralis imaging platform (OCT, fluorescein and ICG angiography, confocal scanning laser ophthalmoscopy)
  • Rodent color fundic imaging (Phoenix Research Labs Micron IV platform)
  • Large animal wide-field, color fundic imaging (Clarity Medical Systems RetCam Shuttle platform)
  • ERG (dark-adapted, light-adapted, flicker responses, ISCEV standards)
  • Indirect and direct ophthalmoscopy, slit-lamp biomicroscopy, corneal fluorescein staining
  • Hackett-McDonald scoring (and customized scoring protocols)
  • Tonometry (intraocular pressure measurements), gonioscopy, Schirmer tear/phenol red thread testing
  • Specialized ocular administration routes (intravitreal, intracameral, subconjunctival, subtenon, subretinal, as well as topical and systemic dose routes)
  • Aqueous/vitreous humor collections; detailed ocular tissue dissections for ocular PK/biodistribution determinations
  • Laser-induced CNV model (minipigs)