In Vitro Services

///In Vitro Services
In Vitro Services 2017-07-04T10:25:21+00:00

Your Partner in In Vitro Research

The cell and molecular biology approach for preclinical research

MPI Research offers quality, customized GLP and non-GLP studies for both the preclinical and clinical phases of the drug development process. Properly designed in vitro methods can determine failure early and help our sponsors optimize subsequent decision-making by narrowing down the drug candidates that can reach the target organ or receptor effectively and safely while delivering the intended pharmacology.

Our in vitro services also extend to the drug discovery process including in vitro drug efficacy, mechanism of action, and DMPK studies which enable our Sponsors to better predict in vivo outcomes

MPI Research provides

  • A fully equipped cell and molecular biology laboratory offering leading-edge technology and expertise
  • Experience with diverse study designs and techniques
  • An impressive record of success in quantitative real-time polymerase chain reaction (qPCR) applications, in vitro toxicity tests, cell-based and biochemical analyses
  • Customized development and GLP validation of cell and molecular based assays including flow cytomertric and qPCR panels.

Service highlights

Our highly qualified cell and molecular biology team brings years of experience from the fields of biopharmaceutical discovery, contract research, and academia to provide you with a full range of advanced in vitro services. Our expertise spans the spectrum of research in drug discovery and development, including

  • Cellular and molecular biology assays
  • In vitro ADME (metabolism, transporters, and binding)
  • In vitro toxicology
  • In vitro support of in vivo studies
  • Biomarkers and gene expression assays
  • Stem cell research

Cellular and Molecular Biology Assays

  • Quantitative real-time PCR (qPCR) and reverse transcription qPCR (qRT-PCR), GLP capable
  • Robotic, fully automated DNA and RNA sample preparation, GLP capable
  • High-throughput flow cytometry, GLP capable
  • Automated, microfluidics-based sizing quantification and quality control of DNA/RNA, and proteins
  • Protein quantification including protein concentration, ELISA, and western blot analysis
  • Microplate assays for enzyme activities and measurement of biomolecules
  • Immunofluorescence assays
  • High-resolution gel molecular imaging

In Vitro ADME

  • Stability
  • Metabolite identification
  • CYP inhibition and induction
  • Non-CYP metabolism
  • Permeability
  • Inhibition
  • Substrate
  • Whole blood partitioning
  • Plasma binding
  • Tissue binding

In Vitro Toxicology

  • High-content imaging analysis for cell proliferation, cytotoxicity, and mitochondria membrane potential
  • CellCiphr® premier
  • Cellomics ToxInsight IVT Platform
  • hERG assay, GLP capable
  • eCiphr® Cardio
  • eCiphr® Neuro
  • Ocular irritation
  • Skin irritation, absorption, and sensitization

In Vitro Support of In Vivo Studies (Biomarkers and Stem Cells)

  • Plasmid- and viral vector-based therapy
    • Human stem cell-based therapy
    • siRNA therapy
    • Vaccines
  • Biomarker and gene expression studies using qRT-PCR and qPCR arrays
  • Single Nucleotide Polymorphism (SNP) genotyping assays
  • Validated immunophenotyping panels for peripheral blood leukocytes evaluation in various species (rat, dog, nonhuman primate, mouse, and swine)
  • Customized assay development in advanced applications, such as rare cell quantification and immune function assessment (up to 10 unique cellular parameters simultaneously detected)
  • Isolation and culture of dendritic cells and Peripheral Blood Mononuclear Cells (PBMCs)